L-arabinose binding protein from Escherichia coli B-r.

Amino acid sequence of the L-arabinose-binding protein from Escherichia coli B/r.

The amino acid sequence of the L-arabinose-binding protein of Escherichia coli B/r was determined by sequenator analyses of reduced and S-pyridylethylated L-arabinose-binding protein and fragments derived by chemical and enzymatic cleavage of the native protein. The fragments were the products of cleavage by cyanogen bromide. BNPS-skatole, hydroxylamine, mild acid hydrolysis, limited trypsin di...

Crystallization and characterization of the L-arabinose-binding protein of Escherichia coli B-r.

The L-arabinose-binding protein was purified by conventional methods from Escherichia coli cell paste and crystallized using Z-methyl-2,4-pentanediol. A molecular weight of 38,000 was determined using both ultracentrifugation and gel filtration. The amino acid composition revealed high concentrations of aspartic and glutamic residues and nonpolar amino acids. The presence of 2 cysteine residues...

Using L-arabinose for Production of Human Growth Hormone in Escherichia coli, Studying the Processing of gIII::hGH Precursor

"In vivo" detection of L-arabinose-binding protein, CRM-negative mutants.

An "in vivo" assay for the detection of mutants negative to CRM (cross-reacting material) is described. l-Arabinose-negative mutants of Escherichia coli B/r were grown on Casamino Acids-l-arabinose plates to which a 3-ml agar layer, containing antiserum to the l-arabinose-binding protein (ABP), had been applied. After incubation and partial lysis of the clones "in situ," the plates were refrige...

The amino acid sequence of the D-galactose-binding protein from Escherichia coli B/r.

The complete primary structure of the Escherichia coli B/r galactose-binding protein was determined by the automated sequencing of fragments produced by cleavage with cyanogen bromide, o-iodosobenzoic acid, limited trypsin digestion, mild acid hydrolysis, and Staphylococcus aureus strain V8 protease. The protein, which has 309 amino acids, is notable in the extent to which it differs from the L...